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SKU: 100ERS

293T Human Kidney Epithelial Cells

Engineered / Reporter / Stable Expression Cells > Kidney & Urinary Cells

Product Type: Human Kidney Cell Lines

Species: Human

Cell Type: Epithelial Cells

Source Organ: Kidney

$1,249.00
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Cell Name: 293T

Tissue: kidney, embryonic; Transformed by: SV40 large T antigen; Original line: 293. Used for transfection assays and as packaging cells. The cell line can be transiently transfected and give extremely high levels of expression of AP fusion proteins. G418 resistant, neomicine resistant. The cell line is known also as HEK293T.

  • Description
    293T is a derivative cell line of the human embryonic kidney cell line HEK293, originally obtained by transforming HEK293 cells with adenovirus 5 DNA, and can be generated by the SV40 T antigen transfection. The 293T cells exhibit epithelial-like morphology and adherent culture characteristics. The stemline karyotype of this cell line is hyperdiploid, with a modal chromosome number of 64. 293T is an aneuploid cell line, displaying chromosomal counts within the near-triploid range. This cell line is tumorigenic and has a high transfection efficiency and is commonly used for lentivirus packaging, gene expression, and signal transduction studies.
  • Product Name
    293T
  • Product Type
    Human Kidney Cell Lines
  • Species
    Human
  • Cell Type
    Epithelial
  • Source Organ
    Kidney
  • Product Type
    Human Kidney Cell Lines
  • Growth Conditions
    37 °C, 5% CO2
  • Application
    293T cells serve as a versatile platform for viral vector production (including retroviruses, lentiviruses, and adenoviruses) by efficiently packaging SV40 origin-containing vectors. Their high transferability supports transient gene expression studies and transfection assay optimization while enabling large-scale recombinant protein production for research/therapeutics. These cells additionally facilitate pseudovirus generation for viral entry/replication studies and drug discovery research targeting ion channels, protein interactions, and post-translational modifications, along with s in RNA interference, cell cycle progression, and cancer metastasis.
  • Size/Quantity
    1 vial
  • Recommended Medium And Supplement

    DMEM + 10% FBS
  • Shipping Conditions
    Dry Ice
  • Storage
    Liquid Nitrogen
  • Biosafety Level
    2

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