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SKU: 0423ATC

B9 Mouse Hybridoma Specialized Cells

Tumor Cell Lines > Myeloma Cell Lines

Product Type: Mouse Myeloma Cell Lines

Species: Mouse- BALB/C; Derived From: B13.29 (First Describ

Cell Type: Other / Specialized Cells

Source Organ: Other

$1,249.00
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Cell Name: B9

Tissue: hybrid, B cells x myeloma; Tumor: hybridoma; Derived from B13.29 (First described as clone 9.9). Fusion partner: SP2/0-Ag14; Non producer hybridoma; IL-6 dependent.

  • Description
    The B9 cell line is a mouse B cell hybridoma, derived from the fusion of BALB/c spleen cells with SP2/0-AG14 myeloma cells. The B9 cells are primarily used for studying immune responses, including cytokine activity. Morphologically, B9 cells are small, round, and adhere to the surface in culture. These cells rely on Interleukin 6 (IL-6) for survival and proliferation and exhibit a dose-dependent response to lipopolysaccharide (LPS) stimulation. They also show sensitivity to hybridoma growth factor (HGF) and do not produce immunoglobulins. B9 cells maintain a stable genetic profile without notable mutations, and no high expression of special antigens or genes has been reported. In terms of tumorigenicity, B9 cells do not exhibit tumorigenic or metastatic properties. It is widely used in immunological assays and hybridoma technology for the study of cytokine function.
  • Product Name
    B9
  • Product Type
    Mouse Myeloma Cell Lines
  • Species
    Mouse- BALB/C; Derived From: B13.29 (First Describ
  • Disease
    Hybridoma
  • Product Type
    Mouse Myeloma Cell Lines
  • Growth Conditions
    37 °C, 5% CO2
  • Application
    The B9 cell line serves as a valuable model for investigating the complex aspects of B cell biology and is used to study IL-6 in inflammatory, autoimmune, and cancer-related diseases. The B9 hybridoma enables a straightforward and highly sensitive assay, known as the B9 bioassay, for assessing IL-6 activity in human, rat, rabbit, pig, and mouse samples. This versatile cell line contributes to our understanding of epidermal regulation during the pathogenesis of various diseases.
  • Size/Quantity
    1 vial
  • Recommended Medium And Supplement

    RPMI 1640 + 10% FBS
  • Shipping Conditions
    Dry Ice
  • Storage
    Liquid Nitrogen
  • Biosafety Level
    1

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