SKU: 449BIC

B95-8 Monkey Blood Fibroblasts Cell Line

Blood and Immune Cells & Products > Lymphoid & Hematopoietic Cell Lines

Product Type: Monkey Lymphocytes Cell Lines

Species: Saguinus oedipus

Cell Type: Fibroblasts

Source Organ: Blood

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Cell Name: B95-8

Cotton-top Tamarin Monkey peripheral blood lymphocyte. Derived from a cotton-top tamarin (Saguinus oedipus). Releases high titres of transforming EBV. Thus it provides a source of EBV to establish continuous lymphocytic lines from human donors. The cells.

  • Description
    The B95-8 cell line was established in 1973 from the peripheral blood lymphocytes of a cotton-top tamarin (Saguinus oedipus), transformed by Epstein-Barr virus (EBV). It is widely used to produce high titers of infectious EBV, facilitating the generation of EBV-transformed human B cell lines. B95-8 cells grow predominantly in suspension with round to oval morphology, occasionally forming small clumps, and around 10% of the cells show slight adherence. These cells carry a near-diploid simian karyotype with chromosomal abnormalities and are EBV-positive but free of HIV-1, HIV-2, HTLV-I/II, HBV, HCV, and CMV. Some batches may contain Type D retrovirus, so screening is recommended. B95-8 cells are responsive to B cell factors like sCD23 and are commonly used in virology and lymphocyte transformation research.
  • Product Name
    B95-8
  • Product Type
    Monkey Lymphocytes Cell Lines
  • Species
    Saguinus oedipus
  • Cell Type
    Fibroblast
  • Source Organ
    Blood
  • Product Type
    Monkey Lymphocytes Cell Lines
  • Growth Conditions
    37 °C, 5% CO2
  • Application
    The B95-8 cell line is a classic model for EBV research, used to reveal the latent infection mechanism of EBV, the oncogenic effects of viral genes (such as LMP-1 and EBNA-2), and the interaction between the virus and host cells. In addition, B95-8 cells can continuously secrete infectious EBV particles, so they are often used as a tool for EBV virus amplification to provide a virus source for the laboratory.
  • Size/Quantity
    1 vial
  • Recommended Medium And Supplement

    RPMI 1640 + 10% FBS
  • Shipping Conditions
    Dry Ice
  • Storage
    Liquid Nitrogen
  • Biosafety Level
    2

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