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SKU: 0649APC

C57BL/6 Mouse Spleen Fibroblasts

Primary Cells > Lymphoid & Immune Tissue Cells

Product Type: Mouse Primary Cells

Species: C57BL/6 Mouse

Cell Type: Fibroblasts

Source Organ: Spleen

$1,249.00
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Cell Name: C57BL/6 Mouse Spleen Fibroblasts

  • Description
    C57BL/6 Mouse Primary Spleen Fibroblasts are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Spleen Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in our Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.
    Mouse Primary Spleen Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by our scientist. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
  • Product Name
    C57BL/6 Mouse Spleen Fibroblasts
  • Product Type
    Mouse Primary Cells
  • Species
    C57BL/6 Mouse
  • Cell Type
    Fibroblast
  • Source Organ
    Spleen
  • Disease
    Normal
  • Product Type
    Mouse Primary Cells
  • Growth Conditions
    37 °C, 5% CO2
  • Size/Quantity
    1 vial
  • Recommended Medium And Supplement
    C57BL/6 Mouse Spleen Fibroblasts Medium Kit
  • Quality Control
    All cells test negative for mycoplasma, bacteria, yeast, and fungi.
  • Shipping Conditions
    Dry Ice
  • Storage
    Liquid Nitrogen
  • Biosafety Level
    1

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