SKU: 0642ATC

F9 Mouse Embryo Specialized Cells

Tumor Cell Lines > Germ Cell Cancer Cell Lines

Product Type: Mouse Teratocarcinoma Cell Lines

Species: Mouse-Male

Cell Type: Other / Specialized Cells

Source Organ: Embryo

Delivery and Shipping

Ship on dry ice.

Cell Name: F9

Description

F9 cells can be stimulated to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cyclic AMP (cAMP); Differentiating cells synthesize plasminogen activator, laminin and type IV collagen; CAMP is active only on cells that have been treated with retinoic acid; Tested and found negative for ectromelia virus (mousepox); Culture vessels must be coated with 0.1% Gelatin prior to use. To do so, cover the surface to of the vessel with 0.1% Gelatin (Difco) in sterile distilled water for 2 hours at 4C, then wash three times with sterile distilled water. Treated flasks and dishes can be stored at room temperatureTumorigenecity: Isoenzyme: Histopathology: teratoma, testicular; Embryonal carcinomaSubculture: Split ratio: Media change: Reverse transcritase: Productionplasminogen activator; Laminin; Type IV collagen.
Product Name

F9
Product Type

Mouse Teratocarcinoma Cell Lines
Species

Mouse-Male
Source Organ

Embryo
Growth Conditions

37 °C, 5% CO2
Size/Quantity

1 vial
Recommended Medium And Supplement

DMEM + 10% FBS
Shipping Conditions

Dry Ice
Storage

Liquid Nitrogen
Biosafety Level

1

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