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SKU: 0065ATC

HEK293 Human Kidney Epithelial Cells

Tumor Cell Lines > Kidney Cancer Cell Lines

Product Type: Human Kidney Cancer Cell Lines

Species: Human

Cell Type: Epithelial Cells

Source Organ: Kidney

$1,249.00
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Cell Name: HEK293

It is established from a human primary embryonal kidney transformed by adenovirus type 5 (Ad 5); Classified as risk category 1 according to the German Central Commission for Biological Safety (ZKBS); Cell line also known as HEK-293 (human embryonic kidney-293).

  • Description
    HEK293 cells are immortalized human embryonic kidney (HEK) cells. Immortalization was achieved by introducing Ad5 E1A and E1B genes, which interfere with cell cycle control and inhibit apoptosis. The presence of Ad5 enables efficient production of recombinant proteins, particularly those driven by the CMV promoter within plasmid vectors. Typically, HEK293 cells exhibit an epithelial morphology. Their size may vary depending on the culturing conditions, with adherent monolayer cultures displaying flatter and larger cells, while cells in suspension culture appear more spheroid. These cells grow well in DMEM supplemented with fetal bovine serum. They are non-tumorigenic and do not exhibit metastatic behavior.
  • Product Name
    HEK293
  • Product Type
    Human Kidney Cancer Cell Lines
  • Species
    Human
  • Cell Type
    Epithelial
  • Source Organ
    Kidney
  • Product Type
    Human Kidney Cancer Cell Lines
  • Growth Conditions
    37 °C, 5% CO2
  • Application
    HEK293 cells have become widely used in various fields due to their convenient growth and transfection capabilities, making them valuable in cancer research. Their high transfection efficiency makes them ideal for producing exogenous proteins and viral vectors for pharmaceutical and biomedical studies. HEK293 cells are particularly useful in transfection experiments involving adenoviral and retroviral vectors, making them essential for vector propagation and related research endeavors.
  • Size/Quantity
    1 vial
  • Recommended Medium And Supplement

    MEM + 2 mM Glutamine + 10% FBS
  • Shipping Conditions
    Dry Ice
  • Storage
    Liquid Nitrogen
  • Biosafety Level
    1

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