SKU: 0096ATC

NCI-H82 Human Lung Cancer Epithelial Cells

Tumor Cell Lines > Lung Cancer Cell Lines

Product Type: Human Lung Cancer Cell Lines

Species: Human

Cell Type: Epithelial Cells

Source Organ: Lung

$1,249.00

Size

1 vial

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Cell Name: NCI-H82

Description

The NCI-H82 cell line was derived by A.F. Gazdar and associates in 1978 from the pleural fluid of a patient with small cell cancer of the lung. The morphology of the original tumor was not characteristic of SCLC. The line is a biochemical and morphological variant of SCLC that expresses neuron specific enolase and the brain isoenzyme of creatine kinase. It does not have detectable levels of L-DOPA decarboxylase or bombesin. The cells produce an abnormally sized p53 mRNA (3.7 Kb). C-myc DNA sequences are amplified about 25 fold, and there is a 24 fold increase in c-myc RNA relative to normal cells. The cells are reported to express functional ANP receptors, but treatment with ANP does not alter their growth pattern. The cells stain positively for neurofilaments and vimentin. There is expression of v-fes, v-fms, Ha-ras, Ki-ras, N-ras and c-raf 1 mRNAs.
Product Name

NCI-H82
Product Type

Human Lung Cancer Cell Lines
Species

Human
Cell Type

Epithelial
Source Organ

Lung
Disease

Cancer
Product Type

Human Lung Cancer Cell Lines
Growth Conditions

37 °C, 5% CO2
Size/Quantity

1 vial
Recommended Medium And Supplement

RPMI 1640 + 10% FBS
Shipping Conditions

Dry Ice
Storage

Liquid Nitrogen
Biosafety Level

1

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