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SKU: 0179ATC

PF-382 Human Pleural Effusion Specialized Cells

Tumor Cell Lines > Leukemia Cell Lines

Product Type: Human Leukemia Cell Lines

Species: Human

Cell Type: Other / Specialized Cells

Source Organ: Effusion

$1,249.00
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Cell Name: PF-382

Tissue: pleural effusion; Tumor: leukemia, T cell; Derived from a patient with acute lymphoblastic leukemia (ALL) in second relapse.

  • Description
    PF-382 is a human T-cell acute lymphoblastic leukemia (T-ALL) cell line established from the pleural effusion of a 6-year-old female patient. The cells exhibit suppressive phenotypic and functional characteristics. Morphologically, PF-382 cells are single, round to oval in shape, and grow in suspension culture. Cytogenetic analysis reveals a modal chromosome number of 46 with a translocation between chromosomes X and 15 (46X,Xq–,15p+). Immunophenotyping shows expression of early T-lymphocyte markers OKT6, Leu-1, Leu-9, and the mature marker OKT8. The cell line lacks CD3 expression and does not form rosettes with sheep erythrocytes. PF-382 cells secrete a 47 kDa inhibitory factor that suppresses the proliferation of myeloid and erythroid progenitor cells. Genetic analyses have identified mutations in several oncogenes and tumor suppressor genes, including TAL1, CREBBP, HRAS, JAK1, NOTCH1, NRAS, PTEN, and TP53.  The tumorigenic potential of PF-382 in vivo has not been characterized.
  • Product Name
    PF-382
  • Product Type
    Human Leukemia Cell Lines
  • Species
    Human
  • Source Organ
    Pleural Effusion
  • Product Type
    Human Leukemia Cell Lines
  • Growth Conditions
    37 °C, 5% CO2
  • Application
    The unique property of PF-382 cells to secrete a soluble factor(s) with the ability to modulate B-cell differentiation makes them an important tool for studying the complex interplay between T-cell subsets and other hematopoietic cell lineages. This cell line holds significant potential as a valuable model system for investigating the regulatory mechanisms of B-cell development and function, providing insights into the broader field of hematopoiesis.
  • Size/Quantity
    1 vial
  • Recommended Medium And Supplement

    RPMI 1640 + 10% FBS
  • Shipping Conditions
    Dry Ice
  • Storage
    Liquid Nitrogen
  • Biosafety Level
    1

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