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SKU: 0310APC

Rat Cardiac Fibroblasts Heart

Primary Cells > Heart & Vascular Cells

Product Type: Rat Primary Cells

Species: Rat

Cell Type: Fibroblasts

Source Organ: Heart

$1,249.00
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Cell Name: Rat Cardiac Fibroblasts

  • Description
    Rat Primary Cardiac Fibroblasts are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Cardiac Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.
    Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Cardiac Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by our scientist. Repeated freezing and thawing of cells is not recommended.
    Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
  • Product Name
    Rat Cardiac Fibroblasts
  • Product Type
    Rat Primary Cells
  • Species
    Rat
  • Cell Type
    Fibroblast
  • Source Organ
    Heart
  • Disease
    Normal
  • Product Type
    Rat Primary Cells
  • Growth Conditions
    37 °C, 5% CO2
  • Size/Quantity
    1 vial
  • Recommended Medium And Supplement
    Rat Cardiac Fibroblasts Medium Kit
  • Quality Control
    All cells test negative for mycoplasma, bacteria, yeast, and fungi.
  • Shipping Conditions
    Dry Ice
  • Storage
    Liquid Nitrogen
  • Biosafety Level
    1

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