SKU: 044ERS

RFP-Human Dopaminergic Neuron Cells

Engineered / Reporter / Stable Expression Cells > Brain & Nervous System Cells

Product Type: Nervous Cells

Species: Human

Cell Type: Neurons

Source Organ: Brain

$1,249.00

Size

1 vial

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Cell Name: RFP-Human Dopaminergic Neuron Cells

Description

Human dopaminergic neuronal cells are isolated from fetal brain tissue obtained from agencies authorized to procure and distribute tissues for research. Dopaminergic neuronal cells are selected in tyrosine free medium supplemented with a mixture of growth factors. Actively growing population of cells are tested for tyrosine hydroxylase (TH) expression by immunocytochemistry. RFP-Expressing Human dopaminergic neuronal cells (RFP-HDaNCs) are selected by puromycin after HDaNCs are infected with lentiviruses expressing RFP. Cells are provided with more than 5×10[5] cells/ vial at passage 2. The cells are grown in regular tissue culture dishes and subcultured at a split ratio of 1 to 2 at confluence. We recommend not to use the cells beyond 4 passages. There may be a significant decrease in the number of tyrosine hydroxylase positive cells after 4 passages as they are not maintained in selective medium to allow the growth of only cells expressing tyrosine hydroxylase.
Product Name

RFP-Human Dopaminergic Neuron Cells
Product Type

Nervous Cells
Species

Human
Cell Type

Neuron
Source Organ

Brain
Disease

Normal
Product Type

Nervous Cells
Growth Conditions

37 °C, 5% CO2
Size/Quantity

1 vial
Recommended Medium And Supplement

RFP-Human Dopaminergic Neuron Cells Medium Kit
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Shipping Conditions

Dry Ice
Storage

Liquid Nitrogen

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